Systems and methods for printer optimization. A method for optimizing a readable dot pattern printed on paper for use with a digital pen is disclosed herein. A test sheet is printed on a selected printer; the first test sheet has a plurality of regions. Each of the regions has a partial digital pattern configured with digitally readable indicia. Each of the indicia has a defined area. An outline is applied to each of the plurality of regions on the test sheet. Ink is applied onto the digital paper using a digital pen within the applied outlines. Data is uploaded from the digital pen to a processor. The smallest indicia readable by the digital pen are determined. A printer control file is updated with the determined smallest indicia.