Invention Grant
US08497067B2 Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
有权
从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途
- Patent Title: Restoration of nucleic acid from degraded or formalin-fixed and paraffin-embedded tissue and uses thereof
- Patent Title (中): 从降解或福尔马林固定和石蜡包埋组织中恢复核酸及其用途
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Application No.: US12087951Application Date: 2007-02-26
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Publication No.: US08497067B2Publication Date: 2013-07-30
- Inventor: Olivier Loudig
- Applicant: Olivier Loudig
- Applicant Address: US NY Bronx
- Assignee: Albert Einstein College of Medicine of Yeshiva University
- Current Assignee: Albert Einstein College of Medicine of Yeshiva University
- Current Assignee Address: US NY Bronx
- Agency: Amster, Rothstein & Ebenstein LLP
- International Application: PCT/US2007/004892 WO 20070226
- International Announcement: WO2007/103018 WO 20070913
- Main IPC: C12P19/34
- IPC: C12P19/34
Abstract:
This invention provides methods, primers and kits for restoration of nucleic acid from tissue, in particular degraded tissue and formalin-fixed and paraffin-embedded (FFPE) tissue, where the methods involve complementary-template reverse-transcription (CT-RT) where short single-stranded DNA sequences reverse-transcribed from mRNA are used for reverse-transcription of complementary sense-RNA templates. The methods can be used to determine patterns of gene expression and chromosomal alterations in archived tissue samples, and may be used to identify expression of disease-related genes.
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