An apparatus (10) for singulating micro-well tape includes a tape drive (12) and a cutting assembly (14). The micro-well tape is advanced into and passes between upper and lower tape retainers (44, 45), with the upper tape retainer (44) including a groove (441) into which a raised ridge (451) of a multiplicity of ridges (450) of the bottom tape retainer (45) extends, with the rows of wells moving between adjacent ridges (450) in a movement direction. When a bar code on the micro-well tape is detected by a sensor 24, a knife blade (30) mounted on a slide block (32) is moved vertically to cut the micro-well tape. The new leading edge of the micro-well tape pushes the cut micro-well tape which is deflected by the raised ridge (451).