A Multiple-Field-Of-View (MFOV) lidar is used to characterize the size and concentration of low concentration of bioaerosol particles. The concept relies on the measurement of the forward scattered light by using the background aerosols at various distances at the back of the sub-visible cloud. It also relies on the subtraction of the background aerosol forward scattering contribution and on the partial attenuation of the first order backscattering. We demonstrate theoretically and experimentally that the MFOV lidar can measure with a good precision the effective diameter of low concentration bioaerosol clouds.