A method for measuring a shafting mechanical fatigue of a turbine generator set, which involves determining a lumped mass model of a turbine generator set and its parameters; calculating a model frequency and a mode shape of the turbine generator set; acquiring an angular velocity change at a machine end; calculating a torque at a calculating section of shafting based on the model frequency, a mode shape curve and an equivalent rigidity of the lumped mass model, so as to obtain a torque-time history curve at the calculating section of the shafting; calculating a fatigue damage accumulative value for a dangerous section of a set shafting under a certain malfunction or disturbance, that is, the shafting mechanical fatigue of the turbine generator set.