Invention Grant
US08507662B2 Methods and kits for reducing non-specific nucleic acid amplification
有权
减少非特异性核酸扩增的方法和试剂盒
- Patent Title: Methods and kits for reducing non-specific nucleic acid amplification
- Patent Title (中): 减少非特异性核酸扩增的方法和试剂盒
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Application No.: US13183480Application Date: 2011-07-15
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Publication No.: US08507662B2Publication Date: 2013-08-13
- Inventor: John Richard Nelson , Robert Scott Duthie , Sonali Jagdish Shah , Clifford Leslie Smith
- Applicant: John Richard Nelson , Robert Scott Duthie , Sonali Jagdish Shah , Clifford Leslie Smith
- Applicant Address: US NY Niskayuna
- Assignee: General Electric Company
- Current Assignee: General Electric Company
- Current Assignee Address: US NY Niskayuna
- Agent Jenifer Haeckl
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N9/00
Abstract:
Methods and kits for efficient amplification of nucleic acids are provided. The methods comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having terminal mismatch primer-dimer structure. The methods also comprise in-vitro amplification of a nucleic acid template employing partially constrained primers having nucleotide analogues. The methods enhance efficiency of nucleic acid amplification reaction by reducing non-specific amplification reactions.
Public/Granted literature
- US20110287510A1 METHODS AND KITS FOR REDUCING NON-SPECIFIC NUCLEIC ACID AMPLIFICATION Public/Granted day:2011-11-24
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