The disclosure concerns a method for optimizing multiresolution blending of confocal microscope images including the steps of: the multi-band blender proceeding pyramidal correlations; the multi-band blender collecting numerical information from each row of a Laplacian image; the multi-band blender integrating the numerical information from a Laplacian domain of pyramid representation; the multi-band blender measuring the discrepancy between adjacent rows; and the multi-band blender optimizing the overlapped region by estimating the best blending coefficients.