Invention Grant
US08510054B2 Intracellular metabolic flux analysis method using substrate labeled with isotope
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使用同位素标记的底物的细胞内代谢通量分析方法
- Patent Title: Intracellular metabolic flux analysis method using substrate labeled with isotope
- Patent Title (中): 使用同位素标记的底物的细胞内代谢通量分析方法
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Application No.: US11048923Application Date: 2005-02-03
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Publication No.: US08510054B2Publication Date: 2013-08-13
- Inventor: Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui
- Applicant: Shintaro Iwatani , Yoshihiro Usuda , Kazuhiko Matsui
- Applicant Address: JP Tokyo
- Assignee: Ajinomoto Co., Inc.
- Current Assignee: Ajinomoto Co., Inc.
- Current Assignee Address: JP Tokyo
- Agency: Nath, Goldberg & Meyer
- Agent Jerald L. Meyer; Sanjana Mangalagiri
- Priority: JP2004-029376 20040205
- Main IPC: G01N33/48
- IPC: G01N33/48 ; C12Q1/00
Abstract:
A method for intracellular metabolic flux analysis comprising (a) culturing cells in a medium not containing any isotope-labeled substrate to a target phase of the metabolic flux analysis, (b) adding an isotope-labeled substrate to the medium, and continuing culture and collecting samples from the medium in time course, (c) measuring isotope distribution in an intracellular metabolite contained in the samples collected in time course, (d) performing a regression analysis for measured data and calculating an isotope distribution ratio in a steady state, and (e) analyzing a metabolic flux of the cultured cells by using the calculated isotope distribution ratio.
Public/Granted literature
- US20050175982A1 Intracellular metabolic flux analysis method using substrate labeled with isotope Public/Granted day:2005-08-11
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