Invention Grant
- Patent Title: Enzymatic antibody processing
- Patent Title (中): 酶抗体加工
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Application No.: US13388002Application Date: 2010-07-28
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Publication No.: US08524470B2Publication Date: 2013-09-03
- Inventor: Markus Haberger , Christine Jung , Dietmar Reusch
- Applicant: Markus Haberger , Christine Jung , Dietmar Reusch
- Applicant Address: US NJ Nutley
- Assignee: Hoffman-La Roche, Inc.
- Current Assignee: Hoffman-La Roche, Inc.
- Current Assignee Address: US NJ Nutley
- Agent Janet M. Martineau
- Priority: EP09166845 20090730
- International Application: PCT/EP2010/004622 WO 20100728
- International Announcement: WO2011/012297 WO 20110203
- Main IPC: C12P21/06
- IPC: C12P21/06 ; A23J1/00

Abstract:
The current invention comprises a method for producing an immunoglobulin or immunoglobulin fragment with defined glycostructure comprising the following steps: a) providing an affinity chromatography column eluate containing the immunoglobulin or immunoglobulin fragment, b) incubating the affinity chromatography column eluate with (α1,3)galactosidase of plant origin, e.g. from green coffee beans (EC 3.2.1.22), c) applying the incubated affinity chromatography column eluate to a protein A chromatography material and recovering the immunoglobulin or immunoglobulin fragment from the protein A chromatography material and thereby producing an immunoglobulin or immunoglobulin fragment with defined glycostructure.
Public/Granted literature
- US20120129216A1 ENZYMATIC ANTIBODY PROCESSING Public/Granted day:2012-05-24
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