Invention Grant
- Patent Title: Culture method for amplifying large numbers of hair follicle stem cells in vitro
- Patent Title (中): 用于体外扩增大量毛囊干细胞的培养方法
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Application No.: US13517068Application Date: 2011-07-20
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Publication No.: US08524492B2Publication Date: 2013-09-03
- Inventor: Jinyu Liu , Yulin Li
- Applicant: Jinyu Liu , Yulin Li
- Applicant Address: CN
- Assignee: Jilin University
- Current Assignee: Jilin University
- Current Assignee Address: CN
- Agency: Banner & Witcoff, Ltd.
- Priority: CN201010238350 20100722
- International Application: PCT/CN2011/001185 WO 20110720
- International Announcement: WO2012/009958 WO 20120126
- Main IPC: C12N5/00
- IPC: C12N5/00
Abstract:
The present invention is a method for amplifying a large numbers of hair follicle stem cells in vitro by using microspheres as carriers for cell culture and a revolving bottle as a fermentation tank for cell proliferation. The method includes digesting hair follicles to obtain hair follicle stem cells, seeding the cells on microcarriers, transferring the microcarriers to a revolving bottle, feeding liquid culture media into the revolving bottle, placing the revolving bottle into a cell incubator, growing the cells on the microcarrier and harvesting the hair follicle stem cells.
Public/Granted literature
- US20120252121A1 Culture Method for Amplifying Large Numbers of Hair Follicle Stem Cells in Vitro Public/Granted day:2012-10-04
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