Solid phase extraction (SPE) disks may be manufactured by providing a suspension comprising glass microfibers and a suspension comprising one or more sorbents and forming a disk by combining the suspensions or by layering the suspensions. This may be followed by a drying procedure to create the finished disk. A disk mold including a collar and plug may be used to establish the size and shape of the disk. Examples of various constructions and processes for forming the disks are provided.