Invention Grant
- Patent Title: Proteases with modified pro regions
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Application No.: US12761275Application Date: 2010-04-15
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Publication No.: US08530218B2Publication Date: 2013-09-10
- Inventor: Eugenio Ferrari , Carol Fioresi , Anita van Kimmenade
- Applicant: Eugenio Ferrari , Carol Fioresi , Anita van Kimmenade
- Applicant Address: US CA Palo Alto
- Assignee: Danisco US Inc.
- Current Assignee: Danisco US Inc.
- Current Assignee Address: US CA Palo Alto
- Agency: Danisco US Inc.
- Main IPC: C12N9/00
- IPC: C12N9/00 ; C12N9/54 ; C12N1/20 ; C12N15/00 ; C07H21/04

Abstract:
The present invention provides methods and compositions for the production of mature proteases in bacterial host cells. The compositions include modified polynucleotides that encode modified proteases, which have at least one mutation in the pro region; the modified serine proteases encoded by the modified polynucleotides; expression cassettes, DNA constructs, and vectors comprising the modified polynucleotides that encode the modified proteases; and the bacterial host cells transformed with the vectors of the invention. The methods include methods for enhancing the production of mature proteases in bacterial host cells e.g. Bacillus sp. host cells. The produced proteases find use in the industrial production of enzymes, suitable for use in various industries, including but not limited to the cleaning, animal feed and textile processing industry.
Public/Granted literature
- US20110045571A1 Proteases With Modified Pro Regions Public/Granted day:2011-02-24
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