A method for dynamical adjusting channel direction and Network-on-Chip architecture thereof are provided. The Network-on-Chip architecture of dynamical adjusting channel direction comprises a first channel, a first router and a second router. The first channel has a first transmission direction. The first router generates and outputs a first output request when receiving a first data. The second router is coupled to the first router through the first channel. The second router receives the first data through the first channel when receiving the first output request.