A method for calibrating a printing machine having a plurality of printing units includes transporting a first group of at-least-semi-transparent sheets at least once through the printing units. A second group of at-least-semi-transparent sheets is transported at least twice through the printing units, with the sheets being turned over between passages. Registration marks are printed on one of the sheets of the first group of sheets, and on one of the sheets of the second group of sheets during their second passage through the printing units. No registration marks are printed on the sheets of the second group of sheets during their first passage through the printing units. The registration marks are measured at a registration sensor located downstream of the printing units. Calibration parameters are determined using the measurements of the first and second registration marks provided by the registration sensor.