The invention relates to a method for determining flow conditions in a measured volume permeated by a fluid spiked with optically detectable particles. A plurality of two-dimensional images of the particle distribution is thereby created at each of a plurality of times, an estimated particle distribution is determined therefrom, and a three-dimensional displacement vector field is calculated. According to the invention, a transfer function for the image detectors used is first determined, by means of which the real distribution is mapped by the image detector. Starting from a roughly estimated initial distribution, and by means of the transfer function, virtual images of the estimated distribution are then calculated and compared to the associated real images. The estimated distribution is modified in an iterative method until sufficient matching of the virtual and real images has been achieved.