Invention Grant
US08546103B2 Method for detecting the presence or absence of pathogenic Staphylococci in a test sample, including test mixture with micro particles 有权
用于检测测试样品中病原体葡萄球菌存在或不存在的方法,包括具有微粒的测试混合物

  • Patent Title: Method for detecting the presence or absence of pathogenic Staphylococci in a test sample, including test mixture with micro particles
  • Patent Title (中): 用于检测测试样品中病原体葡萄球菌存在或不存在的方法,包括具有微粒的测试混合物
  • Application No.: US12823665
    Application Date: 2010-06-25
  • Publication No.: US08546103B2
    Publication Date: 2013-10-01
  • Inventor: Stephen C. Edberg
  • Applicant: Stephen C. Edberg
  • Applicant Address: US FL Sarasota
  • Assignee: Pilots Point, LLC
  • Current Assignee: Pilots Point, LLC
  • Current Assignee Address: US FL Sarasota
  • Main IPC: C12Q1/14
  • IPC: C12Q1/14
Method for detecting the presence or absence of pathogenic Staphylococci in a test sample, including test mixture with micro particles
Abstract:
A presence/absence test for Staphylococcus aureus (S. aureus) involves placing a first generation test sample in a solution that will clot in the presence of S. aureus. The solution contains components that will selectively grow S. aureus and also contains clotting factors that will react with S. aureus, if S. aureus is present in the sample, to clot the solution. Examples of specimen samples that can be tested include nasal swabs and lesion swabs, among others. The test can also be modified to detect the presence or absence of methicillin resistant S. Aureus (MRSA). The addition of micro particles having a size in the range of about 0.1 micron to about 1.0 mm provides localities where the bacteria agglomerate, thereby significantly decreasing the clotting time, and providing a significantly stronger clot. The micro particles can be used in other bacteria tests to accelerate the production of an end result. Such other tests can include a vancomycin-resistant enterococcus test; a Group B Streptococcus test; a test for hemolytic E. coli; and a test for Listeria monocytogenes, to name a few. These tests are all performed in a liquid broth-type reagent mixture and do not necessarily involve clotting of the broth.
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