Invention Grant
US08546125B2 Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
有权
重组宿主和制备聚羟基链烷酸酯的方法
- Patent Title: Recombinant hosts and methods for manufacturing polyhydroxyalkanoate
- Patent Title (中): 重组宿主和制备聚羟基链烷酸酯的方法
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Application No.: US13031350Application Date: 2011-02-21
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Publication No.: US08546125B2Publication Date: 2013-10-01
- Inventor: Guo-Qiang Chen , Zheng-Jun Li , Zhen-Yu Shi , Ying-Ying Guo , Jia Jian , Qiong Wu
- Applicant: Guo-Qiang Chen , Zheng-Jun Li , Zhen-Yu Shi , Ying-Ying Guo , Jia Jian , Qiong Wu
- Applicant Address: CN Tianjin
- Assignee: Tianjin Greenbio Material Co., Ltd.
- Current Assignee: Tianjin Greenbio Material Co., Ltd.
- Current Assignee Address: CN Tianjin
- Agency: Fay Sharpe LLP
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12P9/00 ; C07H21/04

Abstract:
Known attempts using engineered bacteria to produce P(3HB-co-4HB) with carbon sources that are structurally unrelated to 4-hydroxybutyrate resulted in relatively low 4HB monomer content of 1.5 to 5 mol %. The current invention provides recombinant hosts for producing P(3HB-co-4HB) wherein the plasmid including succinate semialdehyde dehydrogenase gene (sucD gene) and 4-hydroxybutyrate dehydrogenase gene (4hbD gene) further includes pyruvate decarboxylase promoter (Ppdc). It was found that the 4HB monomer content in P(3HB-co-4HB) is significantly enhanced to be over 20 mol %, in the range of 8.8 to 23 mol %.
Public/Granted literature
- US20120214213A1 RECOMBINANT HOSTS AND METHODS FOR MANUFACTURING POLYHYDROXYALKANOATE Public/Granted day:2012-08-23
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