Invention Grant
- Patent Title: Prokaryotic RNAi-like system and methods of use
- Patent Title (中): 原核RNAi样系统及其使用方法
-
Application No.: US13055769Application Date: 2009-07-24
-
Publication No.: US08546553B2Publication Date: 2013-10-01
- Inventor: Rebecca Terns , Michael Terns , Caryn Hale
- Applicant: Rebecca Terns , Michael Terns , Caryn Hale
- Applicant Address: US GA Athens
- Assignee: University of Georgia Research Foundation, Inc.
- Current Assignee: University of Georgia Research Foundation, Inc.
- Current Assignee Address: US GA Athens
- Agency: Mueting Raasch & Gebhardt, P.A.
- International Application: PCT/US2009/051745 WO 20090724
- International Announcement: WO2010/011961 WO 20100128
- Main IPC: C07H21/04
- IPC: C07H21/04 ; C12N15/11 ; C12N15/113 ; C12Q1/68
Abstract:
Provided herein are methods for inactivating a target polynucleotide. The methods use a psiRNA having a 5′ region and a 3′ region. The 5′ region includes, but is not limited to, 5 to 10 nucleotides chosen from a repeat from a CRISPR locus immediately upstream of a spacer. The 3′ region is substantially complementary to a portion of the target polynucleotide. The methods may be practiced in a prokaryotic microbe or in vitro. Also provided are polypeptides that have endonuclease activity in the presence of a psiRNA and a target polynucleotide, and methods for using the polypeptides.
Public/Granted literature
- US20110189776A1 PROKARYOTIC RNAi-LIKE SYSTEM AND METHODS OF USE Public/Granted day:2011-08-04
Information query
