Invention Grant
US08551718B2 Functional assay for 5-HT2A, histamine H1 or adrenergic alpha 1B receptors
有权
5-HT2A，组胺H1或肾上腺素α1B受体的功能测定
- Patent Title: Functional assay for 5-HT2A, histamine H1 or adrenergic alpha 1B receptors
- Patent Title (中): 5-HT2A，组胺H1或肾上腺素α1B受体的功能测定
-
Application No.: US13519556Application Date: 2010-03-31
-
Publication No.: US08551718B2Publication Date: 2013-10-08
- Inventor: Ishtiyaque Ahmad , Reddy Venkat Mekala , Reddy Muddukrishna Chillakur , Ramkumar Subramaniam , Jyothsna Ravula , Sriramachandra Murthy Patnala , Ramakrishna Nirogi , Venkateswarlu Jasti
- Applicant: Ishtiyaque Ahmad , Reddy Venkat Mekala , Reddy Muddukrishna Chillakur , Ramkumar Subramaniam , Jyothsna Ravula , Sriramachandra Murthy Patnala , Ramakrishna Nirogi , Venkateswarlu Jasti
- Applicant Address: IN Hyderabad
- Assignee: Suven Life Science Limited
- Current Assignee: Suven Life Science Limited
- Current Assignee Address: IN Hyderabad
- Agency: Iphorgan, Ltd.
- Priority: IN3194/CHE/2009 20091229
- International Application: PCT/IN2010/000209 WO 20100331
- International Announcement: WO2011/080750 WO 20110707
- Main IPC: G01N33/567
- IPC: G01N33/567 ; C12Q1/66
Abstract:
The present invention provides novel functional assay for 5-HT2A, histamine H1 or adrenergic alpha 1b receptors, by measuring intracellular cyclic adenosine monophosphate (cAMP) levels utilizing reporter gene driven cell based assay. The novel assay provides both binding affinity as well as mode of action of compounds in a single set. The novel assay of the invention is useful in identification of compounds acting through 5-HT2A, histamine H1 or adrenergic alpha 1b receptors. Furthermore, the assay offers utility in categorizing compounds in to agonist, partial agonist, inverse agonist and antagonist classes. The novel assay can be scaled up to any high throughput format.
Public/Granted literature
- US20120315657A1 FUNCTIONAL ASSAY FOR 5-HT2A, HISTAMINE H1 OR ADRENERGIC ALPHA 1B RECEPTORS Public/Granted day:2012-12-13
Information query
IPC分类:
