Invention Grant
US08551752B2 RNA polymerase mutant with improved functions 有权
具有改善功能的RNA聚合酶突变体

  • Patent Title: RNA polymerase mutant with improved functions
  • Patent Title (中): 具有改善功能的RNA聚合酶突变体
  • Application No.: US13057418
    Application Date: 2009-08-07
  • Publication No.: US08551752B2
    Publication Date: 2013-10-08
  • Inventor: Seigo OeHiroshi SatoTeruhiko Ide
  • Applicant: Seigo OeHiroshi SatoTeruhiko Ide
  • Applicant Address: JP Yamaguchi
  • Assignee: Tosoh Corporation
  • Current Assignee: Tosoh Corporation
  • Current Assignee Address: JP Yamaguchi
  • Agency: Sughrue Mion, PLLC
  • Priority: JP2008-205491 20080808; JP2009-038979 20090223; JP2009-038980 20090223
  • International Application: PCT/JP2009/064323 WO 20090807
  • International Announcement: WO2010/016621 WO 20100211
  • Main IPC: C12N9/12
  • IPC: C12N9/12
RNA polymerase mutant with improved functions
Abstract:
Disclosed is a T7 RNA polymerase mutant having improved thermal stability and/or specific activity in comparison with wild-type T7-like bacteriophage RNA polymerase, wherein at least one amino acid residue corresponding to at least one of the amino acid residues selected from the group at least consisting of glutamine at position 768, lysine at position 179 and valine at position 685 of the amino acid sequence that composes wild-type T7 RNA polymerase shown in SEQ ID NO: 6, is substituted with another amino acid.
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