A junction field effect transistor structure includes a grid electrode, a source electrode, a drain electrode and a substrate. The grid electrode includes a polysilicon layer and a P-type implanted layer. The source electrode includes an N-type implanted layer, an N-type well layer and a heavy-implanted N-type well layer. The drain electrode includes the N-type implanted layer, the N-type well layer and the heavy-implanted N-type well layer. The substrate is connected with a substrate connecting end by the P-type implanted layer, a P-type well layer, a heavy-implanted P-type well layer and a P-type buried layer. The junction field effect transistor structure of the present invention can be manufactured without adding any masking step based on the existing technologies, and has the high-voltage resistant characteristic to meet the requirements in practical applications. Furthermore, it has the compact structure and compatible technology.