A sample introduction device for reducing carry-over is provided. In the sample introduction device, when a high pressure valve 51 is switched from a load status to an injection status in a total volume injection method, the communication between a stator hole d in communication with an injection port 25 and a stator hole c connected to a flow path toward a separation/detection section 30 occurs earlier than the communication between a stator hole b in communication with a liquid feeder 10 and a stator hole a in communication with a needle 54. A length of a rotor groove Y communicating stator holes d and e or stator holes d and c is set longer than a length of another rotor groove X.