Invention Grant
- Patent Title: Methods for producing hyaluronan in a recombinant host cell
- Patent Title (中): 在重组宿主细胞中产生透明质酸的方法
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Application No.: US13401663Application Date: 2012-02-21
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Publication No.: US08574886B2Publication Date: 2013-11-05
- Inventor: Alan Sloma , Regine Behr , William Widner , Maria Tang , David Sternberg , Stephen Brown
- Applicant: Regine Behr , William Widner , Maria Tang , Stephen Brown , Leslie Naggiar , Linda Sternberg
- Applicant Address: US CA Davis
- Assignee: Novozymes, Inc.
- Current Assignee: Novozymes, Inc.
- Current Assignee Address: US CA Davis
- Agent Robert L. Stames
- Main IPC: C12N1/20
- IPC: C12N1/20 ; C12N15/74 ; C12N15/00 ; C12P19/04 ; C12P19/26 ; C12P19/28 ; C12P1/00 ; C12P21/06 ; C07H21/04

Abstract:
The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase.
Public/Granted literature
- US20120149067A1 Methods for producing hyaluronan in a recombinant host cell Public/Granted day:2012-06-14
Information query
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