A power lock-up setting method and an electronic apparatus using the same are provided. The power lock-up setting method includes following steps. A trigger signal generated by a pressed power switch is received by a pin of a GPIO interface and transmitted to a control unit, such that the control unit starts the electronic apparatus, and a power-on-self-test of a logic processing unit is performed by a processing module. Whether the GPIO interface is set to a power lock-up state is determined by the logic processing unit. If so, a level of the pin is set to a disable level. An operating system is loaded by the processing module to perform an operating system booting process. Accordingly, when the electronic apparatus is under an operating environment of the operating system, the trigger signal is forbidden to be transmitted to the control unit when being generated again.