A gas stop structure capable of repeated inflation and deflation is provided, which includes: a plurality of outer films; a gas chamber area, formed of the plurality of outer films and including a buffer portion and a gas storage portion; a gas stop valve, located between the plurality of outer films, in which a part of the gas stop valve is exposed beyond the plurality of outer films; and a warp portion, connected the buffer portion and the gas storage portion. When a gas tube is placed inside the gas stop valve to inflate the gas chamber area with gas, a height of the inflated gas storage portion is greater than that of the buffer portion to form a sectional difference, so the gas storage portion bends towards the buffer portion to seal the warp portion. After inflation, the gas tube is removed and the gas stop valve is sealed.