In some embodiments, the present disclosure provides a method for producing biofuel from a feedstock that includes one or more coffee sources, such as green coffee beans, whole roasted coffee beans, ground coffee, or spent coffee grounds. Triglycerides and other materials, such as antioxidants, are extracted from the coffee source. In some example, the triglycerides are then transesterified to produce a fatty acid ester biofuel product. In further examples, the method includes obtaining spent coffee grounds from one or more sources, such as residences or businesses that generate spent coffee grounds. The present disclosure also provides biofuels produced using the disclosed method, including mixtures of such biofuels with other fuels, such as other biofuels or petroleum based fuels. Materials obtained from the disclosed method may be put to other uses, such as cosmetics, medicinal products, food products, or combustible materials.