A circuit and method for trimming an internal oscillator of a USB device that generates a clock signal as a frequency source of the USB device detect an end of packet from an input data stream to initialize a counter, identify a token packet in the data stream to detect a start of frame token packet for the counter to carry out clock counting on the clock signal to thereby obtain a count value, and compare the count value with a reference value to determine a trimming code for trimming a clock frequency of the internal oscillator.