Invention Grant
- Patent Title: Detection of nucleic acid sequence differences using coupled ligase detection and polymerase chain reactions
- Patent Title (中): 使用偶联连接酶检测和聚合酶链反应检测核酸序列差异
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Application No.: US13523252Application Date: 2012-06-14
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Publication No.: US08597890B2Publication Date: 2013-12-03
- Inventor: Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
- Applicant: Francis Barany , Matthew Lubin , George Barany , Robert P. Hammer
- Applicant Address: US NY Ithaca
- Assignee: Cornell Research Foundation, Inc.
- Current Assignee: Cornell Research Foundation, Inc.
- Current Assignee Address: US NY Ithaca
- Agency: LeClairRyan, a Professional Corporation
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34
Abstract:
The present invention relates to a method for identifying a target nucleotide sequence. This method involves forming a ligation product on a target nucleotide sequence in a ligation detection reaction mixture, amplifying the ligation product to form an amplified ligation product in a polymerase chain reaction (PCR) mixture, detecting the amplified ligation product, and identifying the target nucleotide sequence. Such coupling of the ligase detection reaction and the polymerase chain reaction permits multiplex detection of nucleic acid sequence difference.
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