A fluorescence analysis system may include a sensor head that has a light source configured to emit light into a flow of fluid, a detector configured to detect fluorescent emissions from the flow of fluid, and a temperature sensor. The system may also include a flow chamber that includes a housing defining a cavity into which the sensor head can be inserted. In some examples, the housing is configured such that, when a flow of fluid enters the housing, the flow of fluid divides into at least a major stream passing adjacent the light source and the detector and a minor stream passing adjacent the temperature sensor. Such a flow chamber may direct fluid past different sensors components while inhibiting a build-up of solids particles, the generation of air locks, or other flow issues attendant with continuous or semi-continuous online operation.