A device, system, and method for passively sealing a vessel containing a fluid and for sampling the fluid without carry-over or cross-contamination between the fluid sampling device, the sealing septum, and the vessel contents. The device includes an actuated septum having a plurality of septum fingers, to passively seal the vessel, and an actuation device, to open the passive seal without carry-over or cross-contamination. Each of the plurality of septum fingers includes a corresponding rib portion. The actuation device can be an actuation ring having an annulus. The plurality of septum fingers and corresponding rib portions are disposed internal or substantially internal to the vessel, while the actuation device is disposed external to the vessel.