Invention Grant
US08623615B2 Solubilization and purification of a target protein fused to a mutant maltose-binding protein
失效
与突变体麦芽糖结合蛋白融合的目标蛋白质的溶解和纯化
- Patent Title: Solubilization and purification of a target protein fused to a mutant maltose-binding protein
- Patent Title (中): 与突变体麦芽糖结合蛋白融合的目标蛋白质的溶解和纯化
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Application No.: US13259837Application Date: 2009-04-01
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Publication No.: US08623615B2Publication Date: 2014-01-07
- Inventor: Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
- Applicant: Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
- Applicant Address: US MA Ipswich
- Assignee: New England Biolabs, Inc.
- Current Assignee: New England Biolabs, Inc.
- Current Assignee Address: US MA Ipswich
- Agency: New England Biolabs, Inc
- Agent Harriet M. Strimpel
- International Application: PCT/US2009/039111 WO 20090401
- International Announcement: WO2010/114532 WO 20101007
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C07K14/00 ; C07H21/02
Abstract:
Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility. The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consists of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
Public/Granted literature
- US20120028305A1 Solubilization and Purification of a Target Protein Fused to a Mutant Maltose-Binding Protein Public/Granted day:2012-02-02
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