A 3D glasses driving method for driving 3D shutter glasses and 3D glasses and a 3D display apparatus using the same, the 3D glasses driving method including: receiving a sync signal from an external device; determining a duty of a driving signal which opens a left eye glass and a right eye glass of the 3D shutter glasses; and opening and closing the left eye glass and the right eye glass of the 3D shutter glasses alternately based on the sync signal and the determined duty. Accordingly, the user is able to watch the 3D image in a descried brightness without experiencing a crosstalk phenomenon.