Image capture with an image capture device that includes an imaging assembly having a tunable spectral response. A default capture setting is applied to the imaging assembly. Preview image data of a scene is captured using the imaging assembly with the default capture setting. A user interface includes a preview image based on the captured preview image data of the scene. A user designation of a region of interest (ROI) in the preview image is accepted, and a user selection of a targeted imaging property for the ROI is accepted. A revised capture setting for the spectral responsivity of the tunable imaging assembly is computed, by revising the default capture setting based on the targeted imaging property for the ROI as selected by the user. The revised capture setting is applied to the imaging assembly. Image data from the imaging assembly is captured using the revised capture setting.