The present invention provides a light engine having four LED light sources in combination with one or more laser light sources. A combination of collimators, bandpass filters, dichroic mirrors, and other elements is operative to direct light from the light sources onto a main optical axis from where it may be focused into a light guide for transport to a instrument or device. Particular embodiments of the invention provide for computer control, intensity control, color control, and light source modulation. The light engine provides light suitable for applications in microscopy, endoscopy, and/or bioanalytical instrumentation.