Invention Grant
- Patent Title: Agent for improving gene transfer efficiency to mammalian cells
- Patent Title (中): 用于提高哺乳动物细胞基因转移效率的药剂
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Application No.: US13583847Application Date: 2011-03-13
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Publication No.: US08633029B2Publication Date: 2014-01-21
- Inventor: Rinji Akada , Mikiko Nakamura
- Applicant: Rinji Akada , Mikiko Nakamura
- Applicant Address: JP Yamaguchi
- Assignee: Yamaguchi University
- Current Assignee: Yamaguchi University
- Current Assignee Address: JP Yamaguchi
- Agency: Chalker Flores, LLP
- Agent Chainey P. Singleton; Edwin S. Flores
- Priority: JP2010-057732 20100315
- International Application: PCT/JP2011/001449 WO 20110313
- International Announcement: WO2011/114678 WO 20110922
- Main IPC: C12N15/88
- IPC: C12N15/88 ; C09K3/00
Abstract:
Provided is an efficiency improving agent for gene transfer to mammalian cells, a method for improving efficiency of gene transfer to mammalian cells, and a method for transforming mammalian cells. The method is characterized in that tRNA is used in combination with a lipofection reagent. Preferably, the agent may be used so that the tRNA concentration in a lipofection solution falls within the range of 3 to 50 μg/mL, and the concentration in a culture is approximately 1/10. More preferably, tRNA and PEG may be used in combination with a lipofection reagent. According to the present invention, gene transfer to mammalian cells with high efficiency can be achieved.
Public/Granted literature
- US20130005041A1 Agent for Improving Gene Transfer Efficiency to Mammalian Cells Public/Granted day:2013-01-03
Information query
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