Example embodiments are directed to methods and apparatuses for generating desired isotopes within water rods of nuclear fuel assemblies. Example methods may include selecting a desired irradiation target based on the target's properties, loading the target into a target rod based on irradiation target and fuel assembly properties, exposing the target rod to neutron flux, and/or harvesting isotopes produced from the irradiation target from the target rod. Example embodiment target rods may house one or more irradiation targets of varying types and phases. Example embodiment securing devices include a ledge collar and/or bushing that support target rods within a water rod and permit moderator/coolant flow through the water rod. Other example embodiment securing devices include one or more washers with one or more apertures drilled therein to hold one or more example embodiment target rods in a water rod while permitting coolant/moderator to flow through the water rod.