The present invention discloses a mobile phone film positioner, comprising a housing, on which a recess for accommodating a mobile phone and mobile phone film is provided, the recess is provided with positioning grooves on two ends thereof, the positioning grooves have projections on the bottoms thereof for positioning the mobile phone film; the present invention further discloses a mobile phone film with positioning structure, to be used with the mobile phone positioner described above, the protective layer of the film is provided with lugs in the corresponding positions thereof, the lugs are provided with positioning holes corresponding to the projections. The positioner and film disclosed in the present invention simplifies the positioning process between the film and screen, making the whole manipulation of film application become simple and time-saving, even the common users could finish the job easily.