A one piece molded vacuum tube holder is fitted with a one piece molded needle hub assembly. One portion of a locking mechanism is provided at the neck of the one piece vacuum tube holder and a second portion of the locking mechanism is provided at the needle hub of the needle hub assembly. The needle hub assembly is press fit to the vacuum tube holder. The one piece vacuum tube holder is configured to have an elongate needle cover integrally extending from its neck. A tamper evident notched joint is provided at the junction where the needle cover is connected to the neck. The opening of the vacuum tube holder through which a conventional vacuum tube is inserted may be sealed for sterilization and transport of the device. A wingnut finger grasp mechanism may be coupled to the needle cover.