Provided is a method for production of bioethanol using watermelon seeds and, more particularly, a method for producing bioethanol with high production yield using watermelon seeds, including: sterilizing watermelon seeds, which are usually discarded as food waste from domestic houses, supermarkets, farm houses, etc., at 121° C. for 10 to 20 minutes under anaerobic conditions; finely grinding the sterilized watermelon seeds; adding glacial acetic acid to the ground seeds to remove linoleic acid therefrom; and inoculating the treated seeds free from linoleic acid with a strain for ethanol fermentation such as Saccharomyces cerevisiae, followed by agitating at 25 to 35° C. and 100 to 300 rpm for 5 to 15 days, to conduct fermentation.