Invention Grant
- Patent Title: Vector for inhibition-based high-throughput screen strategy
- Patent Title (中): 矢量为基于抑制的高通量屏幕策略
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Application No.: US13231436Application Date: 2011-09-13
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Publication No.: US08642327B2Publication Date: 2014-02-04
- Inventor: Wei-Kuang Liu , Min-Pey Ding
- Applicant: Wei-Kuang Liu , Min-Pey Ding
- Applicant Address: TW Tainan County
- Assignee: Scinopharm Taiwan, Ltd.
- Current Assignee: Scinopharm Taiwan, Ltd.
- Current Assignee Address: TW Tainan County
- Agency: Andrews Kurth LLP
- Agent Michael Ye
- Main IPC: C12N15/00
- IPC: C12N15/00
Abstract:
A method for screening cells with high level expression of a target protein is disclosed. The method includes introducing into a plurality of host cells a DNA construct that encodes both a target protein and an inhibitor to an endogenous selectable marker in the host cells, screening host cells harboring the DNA construct for the expression of the endogenous selectable marker, and isolating cells with reduced expression of the selectable marker. Also disclosed is a DNA construct configured to express both the target protein and the inhibitor inside the host cell.
Public/Granted literature
- US20120070891A1 INHIBITION-BASED HIGH-THROUGHPUT SCREEN STRATEGY FOR CELL CLONES Public/Granted day:2012-03-22
Information query
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