Invention Grant
US08642339B2 Culture medium for epithelial stem cells and organoids comprising the stem cells
有权
用于上皮干细胞和包含干细胞的组织的培养基
- Patent Title: Culture medium for epithelial stem cells and organoids comprising the stem cells
- Patent Title (中): 用于上皮干细胞和包含干细胞的组织的培养基
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Application No.: US13147163Application Date: 2010-02-03
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Publication No.: US08642339B2Publication Date: 2014-02-04
- Inventor: Toshiro Sato , Johannes Carolus Clevers
- Applicant: Toshiro Sato , Johannes Carolus Clevers
- Applicant Address: NL Utrecht
- Assignee: Koninklijke Nederlandse Akademie van Wetenschappen
- Current Assignee: Koninklijke Nederlandse Akademie van Wetenschappen
- Current Assignee Address: NL Utrecht
- Agency: Wolf, Greenfield & Sacks, P.C.
- Priority: EP09151970 20090203; EP09171831 20090930
- International Application: PCT/NL2010/000017 WO 20100203
- International Announcement: WO2010/090513 WO 20100812
- Main IPC: C12N5/02
- IPC: C12N5/02
Abstract:
The invention relates to a method for culturing epithelial stem cells, isolated tissue fragments comprising the epithelial stem cells, or adenoma cells, and culturing the cells or fragments in the presence of a Bone Morphogenetic Protein (BMP) inhibitor, a mitogenic growth factor, and a Wnt agonist when culturing epithelial stem cells and isolated tissue fragments. The invention further relates to a cell culture medium comprising a BMP inhibitor, a mitogenic growth factor, and a Wnt agonist, to the use of the culture medium, and to crypt-villus organoids, gastric organoids and pancreatic organoids that are formed in the culture medium.
Public/Granted literature
- US20120028355A1 CULTURE MEDIUM FOR EPITHELIAL STEM CELLS AND ORGANOIDS COMPRISING SAID STEM CELLS Public/Granted day:2012-02-02
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