Invention Grant
US08642755B2 Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
有权
硫代乙酸衍生物在寡核苷酸与苯乙酰二硫化物硫化中的应用
- Patent Title: Use of thioacetic acid derivatives in the sulfurization of oligonucleotides with phenylacetyl disulfide
- Patent Title (中): 硫代乙酸衍生物在寡核苷酸与苯乙酰二硫化物硫化中的应用
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Application No.: US12494493Application Date: 2009-06-30
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Publication No.: US08642755B2Publication Date: 2014-02-04
- Inventor: Agnieszka B. Sierzchala , Douglas J Dellinger , Victor R Mokler , Zoltan Timar
- Applicant: Agnieszka B. Sierzchala , Douglas J Dellinger , Victor R Mokler , Zoltan Timar
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C07H21/00
- IPC: C07H21/00 ; C07D233/58 ; C07C321/12
Abstract:
A method and compositions for sulfurizing at least one phosphite or thiophosphite linkage in an oligonucleotide. The methods employ a phenylacetyl disulfide reagent (known as PADS), phenylthioacetic acid (PTAA) in the presence or absence or N-alkyl imidazole in industrially preferred solvents or solvents that are derived from renewable resources. The use of PTAA eliminates the need to “age” the PADS solution prior to its use in sulfurization reactions.
Public/Granted literature
- US20100331533A1 Use Of Thioacetic Acid Derivatives In The Sulfurization Of Oligonucleotides With Phenylacetyl Disulfide Public/Granted day:2010-12-30
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