An optical connection structure which permits easy and automatic alignment between the optical axes of optical fibers and the optical axes of cores of an optical waveguide, and a production method which ensures that an optical waveguide for the optical connection structure can be efficiently produced with higher dimensional accuracy are provided. An over-cladding layer of the optical waveguide includes an extension portion provided in a longitudinal end portion thereof, and optical fiber fixing grooves are provided in the extension portion as extending along extension lines of cores coaxially with the cores and each having opposite ends, one of which is open in an end face of the extension portion and the other of which is closed. Optical fibers are fitted and fixed in the respective optical fiber fixing grooves. The over-cladding layer further includes a boundary portion (6) provided between the other closed ends of the optical fiber fixing grooves and the cores.