Provided is a process of manufacturing a pneumatic tire, which prevents blisters from being raised on a cylindrical thermoplastic resin film due to an air pocket even in a case where the cylindrical thermoplastic resin film is used as an air permeation preventive layer. Provided is the manufacturing process suitable for a heavy-duty pneumatic tire in particular. The process of manufacturing a pneumatic tire includes: covering the making drum 20 with a cylindrical thermoplastic resin film 6; winding an uncured rubber sheet member 7 as a tie rubber around the thermoplastic resin film 6; and winding a carcass layer 5 around the rubber sheet member 7. The process is characterized in that the rubber sheet member 7 is wound in the circumferential direction of the tire at least twice.