Invention Grant
US08652844B2 Methods for the cryopreservation of animal cells that contain high levels of intracellular lipids
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用于冷冻保存含有高水平细胞内脂质的动物细胞的方法
- Patent Title: Methods for the cryopreservation of animal cells that contain high levels of intracellular lipids
- Patent Title (中): 用于冷冻保存含有高水平细胞内脂质的动物细胞的方法
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Application No.: US12304633Application Date: 2007-06-12
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Publication No.: US08652844B2Publication Date: 2014-02-18
- Inventor: John K. Critser , Hongsheng Men
- Applicant: John K. Critser , Hongsheng Men
- Applicant Address: US MI Columbia
- Assignee: The Curators of the University of Missouri
- Current Assignee: The Curators of the University of Missouri
- Current Assignee Address: US MI Columbia
- Agency: Maginot, Moore & Beck, LLP
- International Application: PCT/US2007/013727 WO 20070612
- International Announcement: WO2007/146253 WO 20071221
- Main IPC: C12N5/00
- IPC: C12N5/00 ; C12N5/02
Abstract:
A method for cryopreservation of animal cells with high level of intracellular lipid content, comprises the steps of conducting a delipation procedure using one or more lipolytic agent(s) and/or lipogenesis inhibitors during culture of the animal cells to stimulate the hydrolysis of intracellular lipids to reduce the lipid content, and vitrifying the treated animal cells using a modified vitrification solution and a modified warming solution.
Public/Granted literature
- US20100190248A1 Methods for the Cryopreservation of Animal Cells that Contain High Levels of Intracellular Lipids Public/Granted day:2010-07-29
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