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US08653326B2 Targeted DNA insertion in plants 有权
植物中靶向的DNA插入

Targeted DNA insertion in plants
Abstract:
Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving “double stranded break” inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.
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