Invention Grant
- Patent Title: Targeted DNA insertion in plants
- Patent Title (中): 植物中靶向的DNA插入
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Application No.: US12497937Application Date: 2009-07-06
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Publication No.: US08653326B2Publication Date: 2014-02-18
- Inventor: Kathleen D′Halluin , Chantal Vanderstraeten , Rene Ruiter
- Applicant: Kathleen D′Halluin , Chantal Vanderstraeten , Rene Ruiter
- Applicant Address: BE Diegem
- Assignee: Bayer Cropscience N.V.
- Current Assignee: Bayer Cropscience N.V.
- Current Assignee Address: BE Diegem
- Agency: Hunton & Williams LLP
- Priority: EP03078700 20031118
- Main IPC: C12N15/82
- IPC: C12N15/82

Abstract:
Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving “double stranded break” inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.
Public/Granted literature
- US20100003759A1 TARGETED DNA INSERTION IN PLANTS Public/Granted day:2010-01-07
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