A microfluidic driving system includes a first planar electrode, a second planar electrode, a third planar electrode, a fourth planar electrode, a power supply unit and a detection module. The second, third and fourth planar electrodes are disposed parallel to the first planar electrode and face-to-face with the first planar electrode to form an accommodation space for accommodating a fluid. An AC power is provided by the power supply unit and an AC electrical field is applied by alternately connecting the third planar electrode and the fourth planar electrode with the first planar electrode for driving the first fluid and the second fluid to flow; and then AC electrical field is also applied by connecting the second planar electrode to the first planar electrode to mix the first fluid and the second fluid. Finally, a detection is performed upon a mixture of the fluids through the detection module.