Invention Grant
US08663924B2 Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls
有权
基于定量PCR的方法来预测使用重复DNA元件（线/正弦）作为阴性对照的下一代测序的靶富集效率
- Patent Title: Quantitative PCR-based method to predict the efficiency of target enrichment for next-generation sequencing using repetitive DNA elements (lines/sines) as negative controls
- Patent Title (中): 基于定量PCR的方法来预测使用重复DNA元件（线/正弦）作为阴性对照的下一代测序的靶富集效率
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Application No.: US13308482Application Date: 2011-11-30
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Publication No.: US08663924B2Publication Date: 2014-03-04
- Inventor: Scott Happe , Joseph Hoang Hai Ong
- Applicant: Scott Happe , Joseph Hoang Hai Ong
- Applicant Address: US CA Santa Clara
- Assignee: Agilent Technologies, Inc.
- Current Assignee: Agilent Technologies, Inc.
- Current Assignee Address: US CA Santa Clara
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C40B30/04 ; C40B40/06 ; C40B40/08
Abstract:
A method for determining an efficiency of target enrichment from a DNA library, includes: adding a negative control sequence and a positive control sequence to the DNA library, or picking a negative control sequence and/or a positive control sequence from the library; determining a pre-capture amount of the negative control sequence and a pre-capture amount of the positive control sequence; performing enrichment of a target sequence from the DNA library using a bait sequence to produce a post-capture library; determining a post-capture amount of the negative control sequence and a post-capture amount of the positive control sequence in the post-capture library; and determining the efficiency of the target enrichment, based on the post-capture amount of the positive control sequence, the post-capture amount of the negative control sequence, the pre-capture amount of the positive control sequence, and the pre-capture amount of the negative control sequence.
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