Invention Grant
US08663948B2 Host, transformant and method for producing the transformant and method for producing O-glycosylated heterologous protein
有权
用于产生转化体的宿主,转化体和方法以及O-糖基化异源蛋白质的制备方法
- Patent Title: Host, transformant and method for producing the transformant and method for producing O-glycosylated heterologous protein
- Patent Title (中): 用于产生转化体的宿主,转化体和方法以及O-糖基化异源蛋白质的制备方法
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Application No.: US13077333Application Date: 2011-03-31
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Publication No.: US08663948B2Publication Date: 2014-03-04
- Inventor: Kaoru Takegawa , Yuko Hama , Hideki Tohda
- Applicant: Kaoru Takegawa , Hideki Tohda , Chihiro Hama
- Applicant Address: JP Tokyo
- Assignee: Asahi Glass Company, Limited
- Current Assignee: Asahi Glass Company, Limited
- Current Assignee Address: JP Tokyo
- Agency: Oblon, Spivak, McClelland, Maier & Neustadt, L.L.P.
- Priority: JP2008-256354 20081001; JP2009-119280 20090515
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12P1/00 ; C12P19/00 ; C12P19/44 ; C12P19/18 ; C12P19/04 ; C12N15/09 ; C12N15/00 ; C12N15/74 ; C12N1/00

Abstract:
The object is to provide a transformant which can produce a heterologous protein having a structurally controlled O-linked sugar chain having an O-Man-Gal disaccharide structure, a method for producing the transformant by using Schizosaccharomyces pombe as the host, and provide a host for producing the transformant and a method for producing an O-glycosylated heterologous protein.An Schizosaccharomyces pombe host having no omh1 gene or an inactivated omh1 gene in its chromosomes for producing an O-glycosylated heterologous protein having an O-linked sugar chain having an O-Man-Gal disaccharide structure by expression of the heterologous protein by a genetic engineering technique and subsequent glycosylation of the expressed heterologous protein. A transformant from the host, a method for producing the transformant and a method for producing an O-glycosylated heterologous protein by using the transformant.
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