Invention Grant
- Patent Title: Fermentation of moderately thermophilic bacilli on sucrose
- Patent Title (中): 中度嗜热杆菌在蔗糖上的发酵
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Application No.: US13384527Application Date: 2010-07-15
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Publication No.: US08663954B2Publication Date: 2014-03-04
- Inventor: Richard Van Kranenburg , Mariska Van Hartskamp
- Applicant: Richard Van Kranenburg , Mariska Van Hartskamp
- Applicant Address: NL Gorinchem
- Assignee: Purac Biochem B.V.
- Current Assignee: Purac Biochem B.V.
- Current Assignee Address: NL Gorinchem
- Agency: Westman, Champlin & Koehler, P.A.
- Agent Peter J. Ims
- Priority: EP09165828 20090717
- International Application: PCT/EP2010/060220 WO 20100715
- International Announcement: WO2011/006966 WO 20110120
- Main IPC: C12P7/56
- IPC: C12P7/56 ; C12P7/40 ; C12P7/06 ; C12P7/26 ; C12P7/18
Abstract:
A method for the construction of a moderately thermophilic Bacillus strain capable of utilizing sucrose as a carbon source includes the transformation of a parent moderately thermophilic Bacillus strain not capable of utilizing sucrose as a carbon source with a polynucleotide comprising a DNA sequence that encodes a polypeptide having sucrose-specific phosphotransferase activity and having i) an amino acid sequence of SEQ ID NO:1 or ii) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:1 and/or comprising a DNA sequence that encodes a polypeptide having sucrose-6-phosphate hydrolase activity and having iii) an amino acid sequence of SEQ ID NO:2 or iv) an amino acid sequence with an identity of at least 70% to the sequence of SEQ ID NO:2.
Public/Granted literature
- US20120208248A1 FERMENTATION OF MODERATELY THERMOPHILIC BACILLI ON SUCROSE Public/Granted day:2012-08-16
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